Eficiencia de Bacillus sp. y Aspergillus sp. en la Degradación de Hidrocarburos

Efficiency of Bacillus Sp. and Aspergillus Sp. in the Degradation of Hydrocarbons

Contenido principal del artículo

Lucero Kassandra Laime Cornejo
Daneiba Hatsi Neyra Encinas
Fernando Antonio Sernaque Auccahuasi

Resumen

El objetivo de la presente investigación fue evaluar la eficiencia de la bacteria Bacillus subtilis y el hongo Aspergillus niger en la degradación de hidrocarburos en Arequipa, 2023, con este enfoque se realizó bajo un tipo de investigación aplicada, con un diseño de investigación experimental puro. Se tomo en cuenta estos aspectos, con el uso de pruebas de laboratorio para poder establecer que tan eficiente fue proceso de degradación de hidrocarburos en 3 muestras: 1 estado solo bacteria, 1 estado solo hongo y 1 estado de la combinación de ambos, en pruebas por triplicado siendo un total de 9 pruebas. Se determino que a través del análisis de varianza de un factor en este caso como único factor el tiempo y a través de Prueba de Tukey lo cual permitieron descubrir que los resultados de las pruebas son significativos, es decir, se determino necesario rechazar la hipótesis nula y aceptar la hipótesis alternativa. Las muestras de bacterias a 72 horas mostraron una degradación de un 99.77% del hidrocarburo, mientras que los hongos a 72 horas mostraron una degradación de un 100% a diferencia de los microorganismos en conjunto que a las 48 horas mostraron 100% de degradación del hidrocarburo (Gasolina) 

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ABBAS, A.S., REFAE, R.I., AMIN, G.A. Y ELSAYED, T.R., 2022. Characterization And Potentials Of Microbial Community In Soil And Water Contaminated With Crude Oil In Egypt. Egyptian Journal Of Chemistry, Vol. 65, No. 8, ISSN 23570245. DOI 10.21608/Ejchem.2022.111269.5063.

AGBAJI, J.E., NWAICHI, E.O. Y ABU, G.O., 2020. Optimization Of Bioremediation-Cocktail For Application In The Eco-Recovery Of Crude Oil Polluted Soil. AAS Open Research, Vol. 3, No. December, ISSN 25159321. DOI 10.12688/Aasopenres.13028.1.

AL-JAWHARI, I.F.H., FLAYYIH, A.I. Y MOHAMMED, D.Q., 2022. Ability Of Pseudomonas Aeruginosa And Bacillus Subtilis To Remove Petroleum Hydrocarbons. International Journal Of Life Science Research Archive, Vol. 3, No. 2, DOI 10.53771/Ijlsra.2022.3.2.0120.

ALVES, T.M., KOKINOU, E., ZODIATIS, G., RADHAKRISHNAN, H., PANAGIOTAKIS, C. Y LARDNER, R., 2016. Multidisciplinary Oil Spill Modeling To Protect Coastal Communities And The Environment Of The Eastern Mediterranean Sea. Scientific Reports [En Línea], Vol. 6, No. 1, ISSN 2045-2322. DOI 10.1038/Srep36882. Disponible En: Https://Www.Nature.Com/Articles/Srep36882.

AO, A. Y AK, A., 2018. Microbial Diversity Of Petroleum Polluted Soil At Ayetoro Community In Ilaje Riverine Oil Producing Areas Of Ondo State, Nigeria. Progress In Petrochemical Science, Vol. 1, No. 5, DOI 10.31031/Pps.2018.01.000525.

BAIG, N. Y SALEH, T.A., 2019. Superhydrophobic Polypropylene Functionalized With Nanoparticles For Efficient Fast Static And Dynamic Separation Of Spilled Oil From Water. Global Challenges [En Línea], Vol. 3, No. 8, ISSN 2056-6646. DOI 10.1002/Gch2.201800115. Disponible En: Https://Onlinelibrary.Wiley.Com/Doi/10.1002/Gch2.201800115.

BEKELE, G.K., GEBRIE, S.A., MEKONEN, E., FIDA, T.T., WOLDESEMAYAT, A.A., ABDA, E.M., TAFESSE, M. Y ASSEFA, F., 2022. Isolation And Characterization Of Diesel-Degrading Bacteria From Hydrocarbon-Contaminated Sites, Flower Farms, And Soda Lakes. International Journal Of Microbiology, Vol. 2022, ISSN 16879198. DOI 10.1155/2022/5655767.

BORDOFF J. Y MCCORMICK, R., 2020. Oil Spill In North Carolina: Implications For The Pipeline Industry. ,

CAPORUSSO, G., GALLO, C. Y TARANTINO, E., 2022. Change Detection Analysis Using Sentinel-1 Satellite Data With SNAP And GEE Regarding Oil Spill In Venezuela. [En Línea]. S.L.: S.N., Pp. 387-404. Disponible En: Https://Link.Springer.Com/10.1007/978-3-031-10545-6_27.

CHAPMAN, S.J., 2018. Review Of Discovering Statistics Using IBM SPSS Statistics, 4th Edition . Journal Of Political Science Education, Vol. 14, No. 1, ISSN 1551-2169. DOI 10.1080/15512169.2017.1366328.

COZZARELLI, I.M., SKALAK, K.J., KENT, D.B., ENGLE, M.A., BENTHEM, A., MUMFORD, A.C., HAASE, K., FARAG, A., HARPER, D., NAGEL, S.C., IWANOWICZ, L.R., OREM, W.H., AKOB, D.M., JAESCHKE, J.B., GALLOWAY, J., KOHLER, M., STOLIKER, D.L. Y JOLLY, G.D., 2017. Environmental Signatures And Effects Of An Oil And Gas Wastewater Spill In The Williston Basin, North Dakota. Science Of The Total Environment [En Línea], Vol. 579, ISSN 00489697. DOI 10.1016/J.Scitotenv.2016.11.157. Disponible En: Https://Linkinghub.Elsevier.Com/Retrieve/Pii/S0048969716326201.

DOMÍNGUEZ, C. Y GÓNZALEZ, R., 2018. Metodología De La Investigación. Madrid: UNED.

EPA, 2006. Guidance On Systematic Planning Using The Data Quality Objectives Process EPA QA / G-4. . S.L.:

FIORELLO, C. V., JODICE, P.G.R., LAMB, J., SATGÉ, Y., MILLS, K. Y ZICCARDI, M., 2021. POSTRELEASE SURVIVAL OF CALIFORNIA BROWN PELICANS (PELECANUS OCCIDENTALIS CALIFORNICUS) FOLLOWING OILING AND REHABILITATION AFTER THE REFUGIO OIL SPILL. Journal Of Wildlife Diseases [En Línea], Vol. 57, No. 3, ISSN 0090-3558. DOI 10.7589/JWD-D-20-00171. Disponible En: Https://Meridian.Allenpress.Com/Jwd/Article/57/3/590/464918/POSTRELEASE-SURVIVAL-OF-CALIFORNIA-BROWN-PELICANS.

FUENTES, S., MÉNDEZ, V., AGUILA, P. Y SEEGER, M., 2014. Bioremediation Of Petroleum Hydrocarbons: Catabolic Genes, Microbial Communities, And Applications. Applied Microbiology And Biotechnology [En Línea], Vol. 98, No. 11, ISSN 0175-7598. DOI 10.1007/S00253-014-5684-9. Disponible En: Http://Link.Springer.Com/10.1007/S00253-014-5684-9.

GONZALES, G.F., 2022. Derrame De Petróleo En Ventanilla, Callao, Enero Del 2022. Revista De La Sociedad Peruana De Medicina Interna [En Línea], Vol. 35, No. 1, ISSN 1609-7173. DOI 10.36393/Spmi.V35i1.658. Disponible En: Http://Revistamedicinainterna.Net/Index.Php/Spmi/Article/View/658.

HALE, R.P. Y SANDBERG, L.A., 2020. Oil, Heartbreak, And Manhood: A (Carbon) Ethnography Of Risk In The Norwegian Petroleum Industry. S.L.: Routledge.

HERNÁNDEZ, R. Y MENDOZA, C., 2018. Metodología De La Investigación - Las Rutas Cuantitativa, Cualitativa Y Mixta [En Línea]. Ciudad De Mexico: S.N. ISBN 9781456223960. Disponible En: Https://Www.Ebooks7-24.Com:443/?Il=6443.,.

KETA, M.N., BELLO, I.M., BAZATA, Y.A., KETA, J.N., AMINU, M., PATRICK, R.J. Y ALIERO, A.S., 2021. Biodegradation Of Crude Oil Contaminated Soils Using Fungal Species Biodegradation Of Crude Oil Contaminated Soils Using Fungal Species. , Vol. 8, No. August,

KHEIRABADI H., GHASSEMI H., IRANI H. Y KOOLIVAND, A., 2018. Environmental Impacts Of The Sanchi Oil Tanker Collision Incident In The East China Sea. Environmental Science & Technology,

LEAL, L., 2008. Experimental Versus Correlational Research. Favorite Activities For The Teaching Of Psychology. [En Línea]. S.L.: S.N., Pp. 20-21. ISBN 1-4338-0349-6; 978-1-4338-0349-9. Disponible En: Http://Search.Ebscohost.Com/Login.Aspx?Direct=True&Db=Psyh&AN=2008-06296-005&Lang=Es&Site=Ehost-Live.

LEEK, J., PENG, R. Y ANDERSON, G., 2017. Design And Analysis Of Experiments. En: A. BISWAS, S. DATTA, J.P. FINE Y M.L. SEGAL (Eds.), Statistical Analysis Of Proteomics, Metabolomics, And Lipidomics Data Using Mass Spectrometry. S.L.: Springer, Pp. 15-45.

LIU, S.-H., ZENG, G.-M., NIU, Q.-Y., LIU, Y., ZHOU, L., JIANG, L.-H., TAN, X., XU, P., ZHANG, C. Y CHENG, M., 2017. Bioremediation Mechanisms Of Combined Pollution Of Pahs And Heavy Metals By Bacteria And Fungi: A Mini Review. Bioresource Technology [En Línea], Vol. 224, ISSN 09608524. DOI 10.1016/J.Biortech.2016.11.095. Disponible En: Https://Linkinghub.Elsevier.Com/Retrieve/Pii/S0960852416316169.

MAHRUL, M.F., RIDWAN, M., JUHARI, M. Y MUSTAFFA, M.I., 2018. Disputed Keystone XL Pipeline Project And Its Implications On Global Energy Security Scenario. Akademia Baru, Vol. 5,

MANCHEGO, H. D. 2021 Bacteria Bacillus Safensis En La Degradación Sobre Hidrocarburos (Gasolina) En Laboratorio Arequipa 2021: Universidad Cesar Vallejo Disponible En: Https://Repositorio.Ucv.Edu.Pe/Bitstream/Handle/20.500.12692/72826/Manchego_MHD-SD.Pdf?Sequence=1&Isallowed=Y

MÁRQUEZ, S.B., 2019. Desarrollo De Nanomateriales/Nanofluidos Para Aplicaciones En Recobro Químico Mejorado De Petróleo. S.L.: Universidad De Granada.

MCCAULEY, D.J., WOODS, P., SULLIVAN, B., BERGMAN, B., JABLONICKY, C., ROAN, A., HIRSHFIELD, M., BOERDER, K. Y WORM, B., 2016. Ending Hide And Seek At Sea. Science, Vol. 351, No. 6278,

MOHR, V. Y GADE, M., 2022. Marine Oil Pollution In An Area Of High Economic Use: Statistical Analyses Of SAR Data From The Western Java Sea. Remote Sensing, Vol. 14, No. 4, ISSN 20724292. DOI 10.3390/Rs14040880.

MOHSIN, M.Z., OMER, R., HUANG, J., MOHSIN, A., GUO, M., QIAN, J. Y ZHUANG, Y., 2021. Advances In Engineered Bacillus Subtilis Biofilms And Spores, And Their Applications In Bioremediation, Biocatalysis, And Biomaterials. Synthetic And Systems Biotechnology [En Línea], Vol. 6, No. 3, ISSN 2405805X. DOI 10.1016/J.Synbio.2021.07.002. Disponible En: Https://Linkinghub.Elsevier.Com/Retrieve/Pii/S2405805X21000387.

MUSA, I.O., JOSHUA, U., IJAH, J. Y ABIOYE, O.P., 2021. Microbial Determination Of Hydrocarbon Polluted Soil In Some Parts Of Niger Microbial Determination Of Hydrocarbon Polluted Soil In Some Parts Of Niger State , Nigeria. , No. November,

NAGGEA, J. Y MILLER, R., 2023. A Comparative Case Study Of Multistakeholder Responses Following Oil Spills In Pointe d’Esny, Mauritius, And Huntington Beach, California. Ecology And Society [En Línea], Vol. 28, No. 1, ISSN 1708-3087. DOI 10.5751/ES-13737-280124. Disponible En: Https://Www.Ecologyandsociety.Org/Vol28/Iss1/Art24/.

NEDBAL, J., GAO, L. Y SUHLING, K., 2020. Bottom-Illuminated Orbital Shaker For Microalgae Cultivation. Hardwarex, Vol. 8, ISSN 24680672. DOI 10.1016/J.Ohx.2020.E00143.

ODILI, U.C., IBRAHIM, F.B., SHAIBU-IMODAGBE, E.M. Y ATTA, H.I., 2020. Comparative Assessment Of Crude Oil Degradation By Monocillium Sp. And Aspergillus Niger. Journal Of Applied Sciences And Environmental Management, Vol. 24, No. 5, ISSN 1119-8362. DOI 10.4314/Jasem.V24i5.13.

OJEWUMI, M.E., ANENIH, E.V., TAIWO, O.S., ADEKEYE, B.T., AWOLU, O.O. Y OJEWUMI, E.O., 2018. A Bioremediation Study Of Raw And Treated Crude Petroleum Oil Polluted Soil With Aspergillus Niger And Pseudomonas Aeruginosa. Journal Of Ecological Engineering, Vol. 19, No. 2, ISSN 22998993. DOI 10.12911/22998993/83564.

PARAGUAY-DELGADO, F., 2020. Técnicas De Microscopía Electrónica Usadas En El Estudio De Nanopartículas. Mundo Nano [En Línea], Vol. 13, No. 25, Disponible En: Https://Www.Scielo.Org.Mx/Pdf/Mn/V13n25/2448-5691-Mn-13-25-101.Pdf.

PARK, S., KIM, Y., LEE, W. Y NAM, C., 2022. Superhydrophobic Polypropylene Sorbent Derived From Discarded Face Masks: A Highly Efficient Adsorbent For Oil Spill Sorbent. Chemosphere [En Línea], Vol. 303, ISSN 00456535. DOI 10.1016/J.Chemosphere.2022.135186. Disponible En: Https://Linkinghub.Elsevier.Com/Retrieve/Pii/S0045653522016794.

PERERA, M., CHINTHAKA, S.D.M., WIJAYARATHNA, C.D., WIJESUNDERA, S., SENEVIRATNE, G. Y JAYASENA, S., 2021. Reduction Of Lag In Crude Oil Degradation By Aspergillus When It Is In Synergy With Bacillus In Biofilm Mode. Bioprocess And Biosystems Engineering [En Línea], Vol. 44, No. 7, ISSN 1615-7591. DOI 10.1007/S00449-021-02534-6. Disponible En: Https://Link.Springer.Com/10.1007/S00449-021-02534-6.

PULIDO CAPURRO, V., ESCOBAR-MAMANI, F., ARANA BUSTAMANTE, C. Y OLIVERA CARHUAZ, E., 2022. Efectos Del Derrame De Petróleo En La Refinería La Pampilla En Las Costas Del Litoral Marino, Lima (Perú). Revista De Investigaciones Altoandinas - Journal Of High Andean Research [En Línea], Vol. 24, No. 1, ISSN 23068582. DOI 10.18271/Ria.2022.411. Disponible En: Https://Huajsapata.Unap.Edu.Pe/Index.Php/Ria/Article/View/411.

QADER, M.Q. Y SHEKHA, Y.A., 2023. Bioremediation Of Heavy Metals By Using Aspergillus Niger And Candida Albicans. ZANCO Journal Of Pure And Applied Sciences,

REZANIA, S., PARK, J., RUPANI, P.F., DARAJEH, N., XU, X. Y SHAHROKHISHAHRAKI, R., 2019. Phytoremediation Potential And Control Of Phragmites Australis As A Green Phytomass: An Overview. Environmental Science And Pollution Research [En Línea], Vol. 26, No. 8, ISSN 0944-1344. DOI 10.1007/S11356-019-04300-4. Disponible En: Http://Link.Springer.Com/10.1007/S11356-019-04300-4.

RODRÍGUEZ, C. Y ALFARO, H., 2013. Metodologia De Investigacion Cientifica Aplicado A La Ingenieria [En Línea]. S.L.: S.N. Disponible En: Http://Www.Unac.Edu.Pe/Documentos/Organizacion/Vri/Cdcitra/Informes_Finales_Investigacion/IF_ABRIL_2012/IF_ALFARO RODRIGUEZ_FIEE.Pdf.

SÁNCHEZ-ROMERO, M.I., GARCÍA-LECHUZ MOYA, J.M., GONZÁLEZ LÓPEZ, J.J. Y ORTA MIRA, N., 2019. Recogida, Transporte Y Procesamiento General De Las Muestras En El Laboratorio De Microbiología. Enfermedades Infecciosas Y Microbiología Clínica [En Línea], Vol. 37, No. 2, ISSN 0213005X. DOI 10.1016/J.Eimc.2017.12.002. Disponible En: Https://Linkinghub.Elsevier.Com/Retrieve/Pii/S0213005X17303907.

SARDUY, Y., 2016. El Análisis De Datos Y Las Investigaciones Cuantitativa Y Cualitativa. Revista Cubana,

SAZANOVA, K. V., FRANK-KAMENETSKAYA, O. V., VLASOV, D.Y., ZELENSKAYA, M.S., VLASOV, A.D., RUSAKOV, A. V. Y PETROVA, M.A., 2020. Carbonate And Oxalate Crystallization By Interaction Of Calcite Marble With Bacillus Subtilis And Bacillus Subtilis–Aspergillus Niger Association. Crystals, Vol. 10, No. 9, ISSN 20734352. DOI 10.3390/Cryst10090756.

STEPANOVA, A.Y., GLADKOV, E.A., OSIPOVA, E.S., GLADKOVA, O.V. Y TERESHONOK, D.V., 2022. Bioremediation Of Soil From Petroleum Contamination. Processes [En Línea], Vol. 10, No. 6, ISSN 2227-9717. DOI 10.3390/Pr10061224. Disponible En: Https://Www.Mdpi.Com/2227-9717/10/6/1224.

THOMAS, G.E., CAMERON, T.C., CAMPO, P., CLARK, D.R., COULON, F., GREGSON, B.H., HEPBURN, L.J., MCGENITY, T.J., MILIOU, A., WHITBY, C. Y MCKEW, B.A., 2020. Bacterial Community Legacy Effects Following The Agia Zoni II Oil-Spill, Greece. Frontiers In Microbiology [En Línea], Vol. 11, ISSN 1664-302X. DOI 10.3389/Fmicb.2020.01706. Disponible En: Https://Www.Frontiersin.Org/Article/10.3389/Fmicb.2020.01706/Full.

UKHUREBOR, K.E., ATHAR, H., ADETUNJI, C.O., AIGBE, U.O., ONYANCHA, R.B. Y ABIFARIN, O., 2021. Environmental Implications Of Petroleum Spillages In The Niger Delta Region Of Nigeria: A Review. Journal Of Environmental Management [En Línea], Vol. 293, ISSN 03014797. DOI 10.1016/J.Jenvman.2021.112872. Disponible En: Https://Linkinghub.Elsevier.Com/Retrieve/Pii/S0301479721009348.

ULE, O., OGBONNA, D.N., OKPARANMA, R.N. Y NRIOR, R.R., 2021. Myco-Enhanced Bioremediation In Open Field Crude Oil Contaminated Soil Using Mucor Racemosus And Aspergillus Niger. Current Journal Of Applied Science And Technology, No. March, DOI 10.9734/Cjast/2021/V40i131241.

WANG, D., LIN, Jiahui, LIN, Junzhang, WANG, W. Y LI, S., 2019. Biodegradation Of Petroleum Hydrocarbons By Bacillus Subtilis BL-27, A Strain With Weak Hydrophobicity. Molecules, Vol. 24, No. 17, ISSN 14203049. DOI 10.3390/Molecules24173021.

WRIGHT, S.K., ALLAN, S., WILKIN, S.M. Y ZICCARDI, M., 2022. Oil Spills In The Arctic. Arctic One Health [En Línea]. Cham: Springer International Publishing, Pp. 159-192. Disponible En: Https://Link.Springer.Com/10.1007/978-3-030-87853-5_7.

YATES, J.S. Y BAKKER, K., 2019. Debating The «Post» In Post-Extractive Economies: Considering The Role Of Oil And Gas In Newfoundland And Labrador’s Imagined Futures. The Extractive Industries And Society,

ZHONG, X., NIU, H., WU, Y., HANNAH, C., LI, S. Y KING, T., 2018. A Modeling Study On The Oil Spill Of M/V Marathassa In Vancouver Harbour. Journal Of Marine Science And Engineering, Vol. 6, No. 3, ISSN 20771312. DOI 10.3390/JMSE6030106.